Payment Terms | L/C, T/T |
Supply Ability | 100,000 per day |
Delivery Time | depending on order quantity |
Packaging Details | Carton Package |
Parameter | FPV FCoV TF GD |
Packing | 8T/box |
Specimen | Feline eye, mouth and nose secretion and other samples |
LOD | 10 copies/μL |
CV | ≤5% |
Applicable | Micgene 242/244, ABI, Bio-Rad |
Brand Name | Biokey |
Model Number | Bik-Q-P036 |
Certification | ISO13485 |
Place of Origin | Guangzhou China |
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Product Specification
Payment Terms | L/C, T/T | Supply Ability | 100,000 per day |
Delivery Time | depending on order quantity | Packaging Details | Carton Package |
Parameter | FPV FCoV TF GD | Packing | 8T/box |
Specimen | Feline eye, mouth and nose secretion and other samples | LOD | 10 copies/μL |
CV | ≤5% | Applicable | Micgene 242/244, ABI, Bio-Rad |
Brand Name | Biokey | Model Number | Bik-Q-P036 |
Certification | ISO13485 | Place of Origin | Guangzhou China |
High Light | Feline Digestive Test Kit PCR ,Taqman Feline Digestive Test Kit ,Taqman Cat GI Test Kit |
Multiple Feline Cat Digestive GI Pathogens Nucleic Acid detection kit PCR Taqman Probe
Intend for Use:
This kit is suitable for the specific detection of feline parvovirus (FPV), feline coronavirus (FCoV), fetal trichomonas (TF) and giardia (GD) in cat eye, mouth and nose secretions and other samples.
Principle:
Under the action of high-efficiency reverse transcriptase, cDNA strands complementary to RNA template were synthesized with PRIMER as starting point and RNA template as template. Choose cat tiny virus, coronavirus, SAN MAO trichomonas fetus, jia drops of giardia conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, and the nucleic acids of feline parvovirus, feline coronavirus, fetal trichomonas and giardia were detected by fluorescence PCR instrument in the completely closed reaction system.
Component:
Composition | Quantity |
FPV/FCoV PCR liquid | 8 tube |
TF/GD PCR liquid | 8 tube |
Positive control | 1 tube |
Negative control | 1tube |
Sample requirements:
1. Sample type
Cat eye, mouth and nose secretion and other samples
2. Sample collection and transportation
Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.
PCR amplification and fluorescence detection (PCR detection area):
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
Step | Cycle number | Temperature | Time |
1 | 1 | 50℃ | 20min |
2 | 1 | 94℃ | 2min30s |
3 | 45 | 94℃ | 10s |
60℃ | 20s collect fluorescent |
FAM and VIC/HEX were selected for detection channel.
Positive judgment value or reference interval:
Results | Ct Value |
Positive | Ct≤38 |
Negative | No Ct value or Ct value=0 |
Gray area | 38<Ct≤45 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Product picture:
Company Details
Business Type:
Manufacturer,Distributor/Wholesaler,Exporter,Seller
Year Established:
2003
Total Annual:
5M-10M
Employee Number:
100~200
Ecer Certification:
Verified Supplier
Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team. &n... Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team. &n...
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