Guangzhou BioKey Healthy Technology Co.Ltd
| Payment Terms | L/C, T/T, Western Union |
| Supply Ability | 100,000 per day |
| Delivery Time | Depending on order quantity |
| Packaging Details | Carton package |
| Package | 24T/Box |
| Validity | 18 months |
| Storage | Store at room temperature, away from light |
| Applicable | Micgene 242 / 244 、ABI QuantStudio5 |
| LoD | 10copies/μL |
| CV | ≤5% |
| Brand Name | Biokey |
| Model Number | BIK-Q-W015 |
| Certification | ISO 13485 |
| Place of Origin | Guangzhou China |
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Product Specification
| Payment Terms | L/C, T/T, Western Union | Supply Ability | 100,000 per day |
| Delivery Time | Depending on order quantity | Packaging Details | Carton package |
| Package | 24T/Box | Validity | 18 months |
| Storage | Store at room temperature, away from light | Applicable | Micgene 242 / 244 、ABI QuantStudio5 |
| LoD | 10copies/μL | CV | ≤5% |
| Brand Name | Biokey | Model Number | BIK-Q-W015 |
| Certification | ISO 13485 | Place of Origin | Guangzhou China |
| High Light | Enterocytozoon Hepatopenaei Pcr Diagnostic Kit ，Pcr Diagnostic Kit Acute Hepatopancreatic Necrosis ，Enterocytozoon Hepatopenaei qpcr test kit | ||
Enterocytozoon Acute hepatopancreatic necrosis syndrome Early mortality syndrome/Enterocytozoon hepatopenaei-18s(EMS/EHP-18S) Nuclear double nucleic acid test kit
Intended use:
This kit is suitable for the specific detection of Enterocytozoon hepatopenaei Acute hepatopancreatic necrosis syndrome Early mortality syndrome /Enterocytozoon hepatopenaei-18s (AHPND/EMS) and hepatoenteric cytiworm -18S (EHP-18S) in adult shrimp, prawn shrimp, shrimp seedlings, bait, processed aquatic products, etc.
Principle:
This kit is chosen prawn acute liver necrosis of pancreas disease and shrimp deeply cell worm - 18 s conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenchable group was separated and fluorescence was emitted, which was detected by fluorescence PCR instrument to realize the detection of acute hepatopancreas necrosis and hepatoenteric cytiworm -18S nucleic acid of shrimp in the completely closed reaction system.
Main components:
| Component | Specification |
| PCR reaction liquid | 24 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Sample requirements:
1. Sample type
Adult shrimp, shrimp, shrimp seedlings, bait, processed aquatic products and other samples
2. Sample collection and transportation
Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.
Using Micgene 242/244/244IVD and ABI QuantStudio5:
PCR amplification and fluorescence Detection (PCR detection area)
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
| Step | Cycle number | Temperature | Time |
| 1 | 1 | 95℃ | 3min |
| 2 | 40 | 95℃ | 10s |
| 60℃ | 30s collect fluorescent |
FAM and VIC/HEX are selected for detection channels.
Result interpretation:
| FAM channel (EMS) | VIC/HEX channel(EHP-18S) | Results judgment | |
| 1 | - | - | The results were reported negative |
| 2 | + | - | The results were reported EMS positive |
| 3 | - | + | The results were reported EHP-18S positive |
| 4 | + | + | The results were reported EMS /EHP-18S positive |
Note: "+" means positive, "-" means negative
The results of interpretation
| Results | Ct Value |
| Positive | Ct≤38 |
| Negative | No Ct value or Ct value=0 |
| Gray area | 38＜Ct≤40 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 40 is considered as
Operation details please check from IFU!
Company Details
Business Type:
Manufacturer,Distributor/Wholesaler,Exporter,Seller
Year Established:
2003
Total Annual:
5M-10M
Employee Number:
100~200
Ecer Certification：
Verified Supplier
Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team. &n... Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team. &n...
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