REAGEN LLC
| Payment Terms | T/T |
| Supply Ability | 100 kits per month |
| Delivery Time | 5-7 days |
| Packaging Details | color packing |
| Type | reagent |
| Size | 96 test |
| Package | color packing |
| Shelf life | one year |
| Cross-Reactivity (%) | 100 |
| Brand Name | REAGEN |
| Model Number | RND99002 |
| Certification | |
| Place of Origin | USA |
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Product Specification
| Payment Terms | T/T | Supply Ability | 100 kits per month |
| Delivery Time | 5-7 days | Packaging Details | color packing |
| Type | reagent | Size | 96 test |
| Package | color packing | Shelf life | one year |
| Cross-Reactivity (%) | 100 | Brand Name | REAGEN |
| Model Number | RND99002 | Certification | |
| Place of Origin | USA | ||
| High Light | Streptomycin Veterinary Residue Test Kit ，ELISA Veterinary Residue Test Kit ，Streptomycin ELISA Test Kit | ||
REAGEN™ Streptomycin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of streptomycin and dihydrostreptomycin in feed, honey, kidney, liver, meat (beef, chicken and pork), milk, serum and urine.
| Sample Type | Detection Limit (ng/g or ppb) |
| Feed | 5 |
| Honey | 2 |
| Meat/Liver/Kidney | 5 |
| Milk | 1 |
| Milk Powder | 1 |
| Serum/Urine | 0.5 |
| Analytes | Cross-reactivity(%) |
| Streptomycin | 100 |
Rapid (10 - 30 minutes), and organic reagent-free extraction methods for various samples with high recovery (75- 115%).
High sensitivity (0.05 ng/g or ppb) and low detection limit (1 ng/g or ppb for meat and milk).
High reproducibility.
A quick ELISA assay (less than 2 hours regardless of number of samples).
The method is based on a competitive colorimetric ELISA assay. The streptomycin antibody has been coated in the plate wells. During the analysis, sample is added along with the streptomycin-horseradish peroxidase conjugate. If the streptomycin residue is present in the sample, it will compete for the streptomycin antibody, thereby preventing the streptomycin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the streptomycin residue concentration in the sample.
Label the individual strips that will be used and aliquot reagents as the following example:
| Component | Volume per Reaction | 24 Reactions |
| Streptomycin Antibody #1 | 50 mL | 1.2 mL |
| HRP-Conjugated Antibody #2 | 50 mL | 1.2 mL |
| 1X Wash Solution | 2.0 mL | 48 mL |
| Stop Buffer | 100 mL | 2.4 mL |
| TMB Substrate | 100 mL | 2.4 mL |
Add 50uL of each Streptomycin Standards in duplicate into different wells (F Add standards to plate only in the order from low concentration to high concentration).
Add 50 uL of each sample in duplicate into different sample wells.
Add 50 uL of HRP-Conjugated Antibody #2 and 50mL Antibody #1 to each well , mix well by gently rocking the plate manually for 1 minute.
Incubate the plate for 30 minutes at room temperature (20 – 25°C / 68 – 77°F) ( F Avoid direct sunlight and cold bench tops during the incubation. Covering the microtiter plate while incubating is recommended).
Wash the plate 5 times with 250 uL of 1X Wash Solution. After the last wash, invert the plate and gently tap the plate dry on paper towels (F Perform the next step immediately after plate washings. Do not allow the plate to air dry between working steps).
Add 100 uL of TMB substrate. Time the reaction immediately after adding the substrate. Mix the solution by gently rocking the plate manually for 1 minute while incubating ( F Do not put any substrate back to the original container to avoid any potential contamination. Any substrate solution exhibiting coloration is indicative of deterioration and should be discarded. Covering the microtiter plate while incubating is recommended).
After incubating for 15 minutes at room temperature (20 – 25°C / 68 – 77°F), add 100 uL of Stop Buffer to stop the enzyme reaction.
Read the plate as soon as possible following the addition of Stop Buffer on a plate reader with 450 nm wavelength (F Before reading, use a lint-free wipe on the bottom of the plate to ensure no moisture or fingerprints interfere with the readings).
Company Details
Business Type:
Manufacturer
Year Established:
2012
Total Annual:
15000000-20000000
Employee Number:
50~80
Ecer Certification：
Verified Supplier
REAGEN INC For more than 13 years REAGEN has been focusing on best-in-class PCR, NGS, ELISA, and lateral flow rapid test kits for human and animal infectious disease diagnostics, as well as for food safety testing. REAGEN develops and manufactures diagnostic kit s for the detection of infectious... REAGEN INC For more than 13 years REAGEN has been focusing on best-in-class PCR, NGS, ELISA, and lateral flow rapid test kits for human and animal infectious disease diagnostics, as well as for food safety testing. REAGEN develops and manufactures diagnostic kit s for the detection of infectious...
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